内脂素(VF)检测试剂盒(酶联免疫吸附试验法,小样本)

Mini Samples ELISA Kit for Visfatin (VF)

Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase

特异性

本试剂盒用于检测内脂素(VF)检测试剂盒(酶联免疫吸附试验法,小样本),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的内脂素(VF)检测试剂盒(酶联免疫吸附试验法,小样本)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本回收率范围(%)平均回收率(%)
serum(n=5)85-9288
EDTA plasma(n=5)80-9481
heparin plasma(n=5)94-10197

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的内脂素(VF)检测试剂盒(酶联免疫吸附试验法,小样本),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中内脂素(VF)检测试剂盒(酶联免疫吸附试验法,小样本)含量的测定值与理论值的比率。

样本1:21:41:81:16
serum(n=5)78-99%97-105%80-98%94-101%
EDTA plasma(n=5)84-104%80-105%91-98%79-101%
heparin plasma(n=5)99-105%86-101%80-96%88-95%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)25µL,37°C温育1小时;
3. 吸弃,加检测溶液A25µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B25µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物25µL,37°C孵育10-20分钟;
8. 加终止液20µL,立即450nm读数。

实验原理

将内脂素(VF)检测试剂盒(酶联免疫吸附试验法,小样本)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的内脂素(VF)检测试剂盒(酶联免疫吸附试验法,小样本)与连接于固相载体上的抗体结合,然后加入生物素化的内脂素(VF)检测试剂盒(酶联免疫吸附试验法,小样本)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的内脂素(VF)检测试剂盒(酶联免疫吸附试验法,小样本)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号适用物种:Homo sapiens (Human,人)应用(仅供研究使用,不用于临床诊断!)
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参考文献

杂志参考文献
Brazilian Journal of BiomoricityEFFECT OF 8 WEEKS ENDURANCE TRAINING ON PLASMA VISFATIN IN MIDDLE-AGED MEN[Brjb: source]
Diabetes Research and Clinical PracticeThe relationship between visfatin and HOMA-IR in hypertensive patients, and the effect of antihypertensive drugs on visfatin and HOMA-IR in hypertensive patients with insulin resistance[ScienceDirect: S0168822711002956]
J Physiol Pharmacol. Early-effect of bariatric surgery (Scopinaro method) on intestinal hormones and adipokines in insulin resistant Wistar rat.[Pubmed: 24304571]
Endocr J.CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes[Pubmed:25168658]
Exp Ther Med.Plasma visfatin levels and mRNA expression of visfatin in peripheral blood mononuclear cells and peripheral blood monocyte-derived macrophages from normal weight females with polycystic ovary syndrome[Pubmed:Pmc3991522]
Eur J Cancer Prev.Visfatin derived from ascites promotes ovarian cancer cell migration through Rho/ROCK signaling-mediated actin polymerization.[Pubmed:25055182]
GenomeVisfatin −948G/T and resistin −420C/Gpolymorphisms in Egyptian type 2 diabetic patients with and without cardiovascular diseases[Nrcresearchpress:Source]
PLOS ONEAntidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting Drug[PubMed: 26262991]
Alcoholism and Drug AddictionChanges in concentration of visfatin during four weeks of inpatient treatment of alcohol dependent males[Science: Article]
CytokineVariation in blood levels of hormones in obese patients following weight reduction induced by endoscopic and surgical bariatric therapies[PubMed: 26539806]
GenomeGenetic Variations in Visfatin and Resistin Genes in Egyptian Type 2 Diabetic Patients With and Without Complications[PubMed: 25120107]
Acta HistochemicaFavorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines[science:S0065128116301234]
Endocrine, Metabolic & Immune Disorders-Drug TargetsChanges in Serum Nampt Levels and Its Significances in Diabetic Nephropathy Patients-The Potential Role of Nampt in T2DM with Diabetic Nephropathy.[pubmed:28699485]
Endocrine ConnectionsPost-chemotherapy serum anti-müllerian hormone level predicts ovarian function recovery[Pubmed:30012648]
Bioscience ReportsEffect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats[Pubmed:29784871]
ContraceptionHyperinsulinemic-euglycemic clamp over the first year of use of depot-medroxyprogesterone acetate as a contraceptive[Pubmed:29665358]
Cranio - Journal of Craniomandibular PracticeAnalysis of synovial fluid visfatin level in temporomandibular joint disorders[Pubmed:29385907]
NutrireResistin and visfatin concentrations are related to central obesity and inflammation in Brazilian children[10.1186:s41110-018-0060-7]
DNA and Cell BiologyTranscriptional Regulation of NAMPT Gene by Glycogen Synthase Kinase 3β in Goat Adipocytes[Pubmed: 30615481]
Jundishapur Journal of Chronic Disease CareThe Effect of 8 Weeks Aquatic Exercise Program on Plasma Visfatin Level and Some Blood Factors in Obese Men[]
Biomarkers in Heart Failure and Associated DiseasesAdipokines as Biomarkers in Health and Disease[]
Journal of Translational MedicinePlasma concentration and expression of adipokines in epicardial and subcutaneous adipose tissue are associated with impaired left ventricular filling pattern[Pubmed: 31533725]
clinical neurology and neurosurgeryRelationshıp of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levels[Pubmed: 32311618]
The Horizon of Medical SciencesEffects of 10-Week Concurrent Training on Insulin Resistance and the Serum Levels of Vaspin and Visfatin in Overweight Females[]
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGYDifferent Ratios of Corn and Coconut Oil Blends in High©\Fat Diets Influence Fat Deposition without Altering Metabolic Biomarkers in Male Rats[]