抗血管紧张素转化酶2抗体(Anti-ACE2)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Anti-Angiotensin I Converting Enzyme 2 Antibody (Anti-ACE2)
ACEH; ACEII; ACE-II; Peptidyl-Dipeptidase A; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; Metalloprotease MPROT15
产品包装(模拟)
产品包装(模拟)
实验结果图
标准曲线图
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测抗血管紧张素转化酶2抗体(Anti-ACE2)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的抗血管紧张素转化酶2抗体(Anti-ACE2)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 82-101 | 95 |
| EDTA plasma(n=5) | 86-103 | 89 |
| heparin plasma(n=5) | 79-94 | 84 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的抗血管紧张素转化酶2抗体(Anti-ACE2)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中抗血管紧张素转化酶2抗体(Anti-ACE2)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-90% | 96-103% | 97-104% | 94-101% |
| EDTA plasma(n=5) | 94-101% | 79-97% | 80-102% | 93-104% |
| heparin plasma(n=5) | 80-88% | 91-98% | 80-104% | 80-99% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100μL，37°C孵育1小时；
4. 洗板5次；
5. 加TMB底物90µL，37°C孵育10-20分钟；
6. 加终止液50µL，立即450nm读数。
实验原理
将抗原包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的抗血管紧张素转化酶2抗体(Anti-ACE2)与连接于固相载体上的抗原结合，然后加入HRP标记的二抗，彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗血管紧张素转化酶2抗体(Anti-ACE2)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| EPB886Hu61 | 血管紧张素转化酶2(ACE2)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB886Hu01 | 血管紧张素转化酶2(ACE2)活性蛋白 | Cell culture; Activity Assays. |
| APB886Hu61 | 血管紧张素转化酶2(ACE2)活性蛋白 | Cell culture; Activity Assays. |
| RPB886Hu01 | 血管紧张素转化酶2(ACE2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB886Hu02 | 血管紧张素转化酶2(ACE2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB886Hu02 | 血管紧张素转化酶2(ACE2)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB886Hu01 | 血管紧张素转化酶2(ACE2)多克隆抗体 | WB |
| MAB886Hu23 | 血管紧张素转化酶2(ACE2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB886Hu24 | 血管紧张素转化酶2(ACE2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB886Hu25 | 血管紧张素转化酶2(ACE2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB886Hu21 | 血管紧张素转化酶2(ACE2)单克隆抗体 | WB; IHC |
| MAB886Hu22 | 血管紧张素转化酶2(ACE2)单克隆抗体 | WB; IHC |
| FAB886Hu08 | 抗血管紧张素转化酶2(ACE2)单克隆抗体 | Flow cytometry. |
| FAB886Hu04 | 抗血管紧张素转化酶2(ACE2)单克隆抗体 | Flow cytometry. |
| FAB886Hu02 | 抗血管紧张素转化酶2(ACE2)单克隆抗体 | Flow cytometry. |
| FAB886Hu06 | 抗血管紧张素转化酶2(ACE2)单克隆抗体 | Flow cytometry. |
| SEB886Hu | 血管紧张素转化酶2(ACE2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEB886Hu | 抗血管紧张素转化酶2抗体(Anti-ACE2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| LMB886Hu | 血管紧张素转化酶2(ACE2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Nature Medicine | Angiotensin-Converting Enzyme 2 Overexpression Remarkably Ameliorated Glomerular Injury in a Rat Model of Diabetic Nephropathy: A Comparison with ACE Inhibition[MolMed: 10_11_liu] |
| Journal of Cancer Therapy | Plasma Levels of Angiotensin-Converting Enzymes 1 and 2 and AGTR2 (T1247G and A5235G) Gene Polymorphisms Are Associated to Breast Cancer Progression.[Scirp: Source] |
| Reprod Toxicol.? | ACE2 activation by xanthenone prevents leptin-induced increases in blood pressure and proteinuria during pregnancy in Sprague-Dawley rats[Pubmed:25205467] |
| Intensive Care Medicine Experimental | Angiotensin converting enzymes in patients with acute respiratory distress syndrome[Content: 3] |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis[S0021967317311858] |
| Journal of Clinical Pharmacy and Therapeutics | Relationship between genetic variants of ACE2 gene and circulating levels of ACE2 and its metabolites.[pubmed:28895159] |
| Innate Immunity | Lipoxin A attenuates LPS-induced acute lung injury via activation of the ACE2-Ang-(1-7)-Mas axis[Pubmed:29969931] |
| Journal of Surgical Research | Impact of angiotensin-converting enzyme 2 levels on postoperative pneumonia after esophagectomy[Pubmed:29506841] |
| canadian journal of physiology and pharmacology | Could cardioprotective effect of ACE2 activator “diminazene aceturate” is more potent than ACE inhibitor “Enalapril” on acute myocardial infarction in rats?[Pubmed: 30840489] |
| Intensive Care Medicine | Angiotensin-converting enzymes in acute respiratory distress syndrome[] |
| BioMed Research International | AVE 0991 Attenuates Pyroptosis and Liver Damage after Heatstroke by Inhibiting the ROS-NLRP3 Inflammatory Signalling Pathway[] |
| Heart and vessels | Relationship between circulating levels of angiotensin-converting enzyme 2-angiotensin-(1–7)-MAS axis and coronary heart disease[Pubmed: 31359146] |
| Medicine | Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study[Pubmed: 32756181] |
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| Clinical Research in Cardiology | Determinants of soluble angiotensin-converting enzyme 2 concentrations in adult patients with complex congenital heart disease[33280062] |
| Clinical & Translational Immunology | Pattern of circulating SARS©\CoV©\2©\specific antibody©\secreting and memory B©\cell generation in patients with acute COVID©\19[33552508] |
| researchsquare | Novel transgenic mice with Cre-dependent co-expression of GFP and human ACE2: a safe tool for study of COVID-19 pathogenesis[33855640] |
| Viruses | Different Neutralization Sensitivity of SARS-CoV-2 Cell-to-Cell and Cell-Free Modes of Infection to Convalescent Sera[] |
| Sci Rep | Immunoreactivity of the SARS-CoV-2 entry proteins ACE-2 and TMPRSS-2 in murine models of hormonal manipulation, ageing, and cardiac injury[34907257] |
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| Metabolites | Ramipril Reduces Acylcarnitines and Distinctly Increases Angiotensin-Converting Enzyme 2 Expression in Lungs of Rats[Pubmed:35448480] |
