基质金属蛋白酶1(MMP1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Matrix Metalloproteinase 1 (MMP1)
CLGN; CLG1; Collagenase; Interstitial Collagenase; Vertebrate Collagenase; Fibroblast Collagenase
- 编号SEA097Hu
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.156-10ng/mL
- 灵敏度最小可检测剂量小于等于0.059ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载英文说明书 中文说明书
- 规格48T96T96T*596T*1096T*100
- 价格¥ 2646¥ 3780¥ 17010¥ 32130¥ 264600
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产品包装(模拟)
产品包装(模拟)
实验结果图
标准曲线图
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测基质金属蛋白酶1(MMP1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的基质金属蛋白酶1(MMP1)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 86-103 | 94 |
| heparin plasma(n=5) | 85-92 | 88 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的基质金属蛋白酶1(MMP1)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中基质金属蛋白酶1(MMP1)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-98% | 84-93% | 80-96% | 85-93% |
| heparin plasma(n=5) | 80-101% | 94-102% | 81-88% | 87-95% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将基质金属蛋白酶1(MMP1)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的基质金属蛋白酶1(MMP1)与连接于固相载体上的抗体结合，然后加入生物素化的基质金属蛋白酶1(MMP1)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的基质金属蛋白酶1(MMP1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA097Hu01 | 基质金属蛋白酶1(MMP1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA097Hu61 | 基质金属蛋白酶1(MMP1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA097Hu61 | 基质金属蛋白酶1(MMP1)活性蛋白 | Cell culture; Activity Assays. |
| PAA097Hu01 | 基质金属蛋白酶1(MMP1)多克隆抗体 | WB; IHC |
| LAA097Hu71 | 基质金属蛋白酶1(MMP1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA097Hu81 | 基质金属蛋白酶1(MMP1)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA097Hu22 | 基质金属蛋白酶1(MMP1)单克隆抗体 | WB; IHC; ICC; IP. |
| MEA097Hu | 基质金属蛋白酶1(MMP1)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA097Hu | 基质金属蛋白酶1(MMP1)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA097Hu | 基质金属蛋白酶1(MMP1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA097Hu | 基质金属蛋白酶1(MMP1)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA097Hu | 基质金属蛋白酶1(MMP1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA097Hu01 | 基质金属蛋白酶1(MMP1)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Parasitology Research | Therapeutic effect of alpha lipoic acid combined with praziquantel on liver fibrosis induced by Schistosoma mansoni challenged mice[SpringerLink: j33n80517577535k] |
| Infection and Immunity | Bovine Respiratory Syncytial Virus and Histophilus somni Interaction at the Alveolar Barrier[Pubmed: 23649093] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches[Plosone: Source] |
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke[Pubmed:24671655] |
| The Journal of Pathology | Mycobacterium tuberculosis dysregulates MMP/TIMP balance to drive rapid cavitation and unrestrained bacterial proliferation[Pubmed:25186281] |
| Connective Tissue Research | Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes[Pubmed:25333747] |
| J Immunol | Heme Oxygenase-1 Regulation of Matrix Metalloproteinase-1 Expression Underlies Distinct Disease Profiles in Tuberculosis[PubMed: 26268658] |
| Cellular & Molecular Immunology | Effect of ebosin on modulating interleukin-1&bgr;-induced inflammatory responses in rat fibroblast-like synoviocytes[PubMed: 25938977] |
| J Periodontal Implant Sci | Assessment of MMP-1, MMP-8 and TIMP-2 in experimental periodontitis treated with kaempferol[pmc:PMC4848383] |
| Int Wound J. | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases.[Pubmed:26991748] |
| Cellular & Molecular Immunology | Effect of ebosin on modulating interleukin-1β-induced inflammatory responses in rat fibroblast-like synoviocytes[pubmed:25938977] |
| Molecular Medicine Reports | Icariin inhibits MMP‑1, MMP‑3 and MMP‑13 expression through MAPK pathways in IL‑1β‑stimulated SW1353 chondrosarcoma cells[pubmed:28447732] |
| Analytical Methods | The development of a matrix metalloproteinase-1 biosensor based on the surface plasmon resonance imaging technique[c6ay01856d] |
| Journal of Cellular Physiology | Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models[pubmed:27739057] |
| Journal of Applied Animal Research | Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts:an in vitro approach to bovine digital dermatitis [10.108:09712119.2017.1335208] |
| Analytical Methods | SPRI biosensors for quantitative determination of matrix metalloproteinase-2[DOI:10.1039/C7AY00786H] |
| International Wound Journal | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases[pubmed:26991748] |
| European Heart Journal | Biomarker‐based phenotyping of myocardial fibrosis identifies patients with heart failure with preserved ejection fraction resistant to the beneficial effects of …[Pubmed:29709099] |
| Journal of Agricultural and Food Chemistry | Hawthorn polyphenol extract inhibits UVB-induced skin photoaging by regulating MMP expression and type I procollagen production in mice[Pubmed:30032605] |
| Oral Diseases | TIMP‐1 association with collagen type I overproduction in hereditary gingival fibromatosis[Pubmed:29989318] |
| Journal of Applied Animal Research | Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an approach to bovine digital dermatitis[10.1080:09712119.2017.1335208] |
| PLoS One | Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis[Pubmed: 30939166] |
| МАТРИКСНЫЕ МЕТАЛЛОПРОТЕИНАЗЫ: ЗНАЧЕНИЕ В РЕМОДЕЛИРОВАНИИ СЕРДЦА У ПАЦИЕНТОВ С ДИСПЛАЗИЕЙ СОЕДИНИТЕЛЬНОЙ ТКАНИ[] | |
| International Journal of Molecular Sciences | A New Analytical Method for Determination of Cathepsin L Based on the Surface Plasmon Resonance Imaging Biosensor[Pubmed: 31052424] |
| INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY | MT 1‐MMP evaluation in neointimal hyperplasia in the late follow‐up after prosthesis implantation[] |
| Journal of Plastic, Reconstructive & Aesthetic Surgery | Intraluminal fluid infusion in a rat jejunum ischemia/reperfusion model is associated with improved tissue perfusion and less mucosal damage[Pubmed: 31734236] |
| Bone Research | Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis[Pubmed: 32047705] |
| Scientific Reports | Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis[Pubmed: 31980722] |
| JOURNAL OF ETHNOPHARMACOLOGY | Danlou tablet inhibits the inflammatory reaction of high-fat diet-induced atherosclerosis in ApoE knockout mice with myocardial ischemia via the NF-κB signaling …[Pubmed: 32745509] |
| Food Funct | Undenatured type II collagen prevents and treats the osteoarthritis and motor function degradation in T2DM patients and db/db mice[33890588] |
| Phytomedicine | Transcriptomic profiling revealed the role of apigenin-4′-O-α-L-rhamnoside in inhibiting the activation of rheumatoid arthritis fibroblast-like synoviocytes via MAPK …[Pubmed:35660352] |
| Journal of Immunology Research | Ebosin Attenuates the Inflammatory Responses Induced by TNF-α through Inhibiting NF-κB and MAPK Pathways in Rat Fibroblast-Like Synoviocytes[Pubmed:35340587] |
