基质金属蛋白酶1(MMP1)真核蛋白
Eukaryotic Matrix Metalloproteinase 1 (MMP1)
CLGN; CLG1; Collagenase; Interstitial Collagenase; Vertebrate Collagenase; Fibroblast Collagenase
- 编号EPA097Hu61
 - 物种Homo sapiens (Human,人)相同的名称,不同的物种。
 - 来源真核表达
 - 宿主CHO Cell
 - 内毒素水平<1.0EU/µg(LAL法测定)
 - 亚细胞定位细胞外基质
 - 预测分子量53.5kDa
 - 实际分子量58&62kDa(差异分析请参阅说明书)
 - 片段与标签Phe20~Asn469 with N-terminal His Tag
 - 缓冲液成份20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
 - 性状冻干粉
 - 纯度> 95%
 - 等电点6.9
 - 应用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,请参见活性蛋白。 - 下载英文说明书 中文说明书
 - 规格10µg50µg200µg1mg5mg
 - 价格¥ 2880¥ 7200¥ 14400¥ 43200¥ 108000
 - 欲了解实际交易价格和更多情况,请与当地经销商联系!
 
序列

用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) | 
| RPA097Hu01 | 基质金属蛋白酶1(MMP1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. | 
| EPA097Hu61 | 基质金属蛋白酶1(MMP1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. | 
| APA097Hu61 | 基质金属蛋白酶1(MMP1)活性蛋白 | Cell culture; Activity Assays. | 
| PAA097Hu01 | 基质金属蛋白酶1(MMP1)多克隆抗体 | WB; IHC | 
| LAA097Hu71 | 基质金属蛋白酶1(MMP1)多克隆抗体(生物素标记) | WB; IHC; ICC. | 
| LAA097Hu81 | 基质金属蛋白酶1(MMP1)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. | 
| MAA097Hu22 | 基质金属蛋白酶1(MMP1)单克隆抗体 | WB; IHC; ICC; IP. | 
| MEA097Hu | 基质金属蛋白酶1(MMP1)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. | 
| HEA097Hu | 基质金属蛋白酶1(MMP1)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. | 
| SEA097Hu | 基质金属蛋白酶1(MMP1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. | 
| SCA097Hu | 基质金属蛋白酶1(MMP1)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. | 
| LMA097Hu | 基质金属蛋白酶1(MMP1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. | 
| KSA097Hu01 | 基质金属蛋白酶1(MMP1)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 | 
参考文献
| 杂志 | 参考文献 | 
| Parasitology Research | Therapeutic effect of alpha lipoic acid combined with praziquantel on liver fibrosis induced by Schistosoma mansoni challenged mice[SpringerLink: j33n80517577535k] | 
| Infection and Immunity | Bovine Respiratory Syncytial Virus and Histophilus somni Interaction at the Alveolar Barrier[Pubmed: 23649093] | 
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches[Plosone: Source] | 
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke[Pubmed:24671655] | 
| The Journal of Pathology | Mycobacterium tuberculosis dysregulates MMP/TIMP balance to drive rapid cavitation and unrestrained bacterial proliferation[Pubmed:25186281] | 
| Connective Tissue Research | Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes[Pubmed:25333747] | 
| J Immunol | Heme Oxygenase-1 Regulation of Matrix Metalloproteinase-1 Expression Underlies Distinct Disease Profiles in Tuberculosis[PubMed: 26268658] | 
| Cellular & Molecular Immunology | Effect of ebosin on modulating interleukin-1&bgr;-induced inflammatory responses in rat fibroblast-like synoviocytes[PubMed: 25938977] | 
| J Periodontal Implant Sci | Assessment of MMP-1, MMP-8 and TIMP-2 in experimental periodontitis treated with kaempferol[pmc:PMC4848383] | 
| Int Wound J. | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases.[Pubmed:26991748] | 
| Cellular & Molecular Immunology | Effect of ebosin on modulating interleukin-1β-induced inflammatory responses in rat fibroblast-like synoviocytes[pubmed:25938977] | 
| Molecular Medicine Reports | Icariin inhibits MMP‑1, MMP‑3 and MMP‑13 expression through MAPK pathways in IL‑1β‑stimulated SW1353 chondrosarcoma cells[pubmed:28447732] | 
| Analytical Methods | The development of a matrix metalloproteinase-1 biosensor based on the surface plasmon resonance imaging technique[c6ay01856d] | 
| Journal of Cellular Physiology | Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models[pubmed:27739057] | 
| Journal of Applied Animal Research | Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts:an in vitro approach to bovine digital dermatitis [10.108:09712119.2017.1335208] | 
| Analytical Methods | SPRI biosensors for quantitative determination of matrix metalloproteinase-2[DOI:10.1039/C7AY00786H] | 
| International Wound Journal | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases[pubmed:26991748] | 
| European Heart Journal | Biomarker‐based phenotyping of myocardial fibrosis identifies patients with heart failure with preserved ejection fraction resistant to the beneficial effects of …[Pubmed:29709099] | 
| Journal of Agricultural and Food Chemistry | Hawthorn polyphenol extract inhibits UVB-induced skin photoaging by regulating MMP expression and type I procollagen production in mice[Pubmed:30032605] | 
| Oral Diseases | TIMP‐1 association with collagen type I overproduction in hereditary gingival fibromatosis[Pubmed:29989318] | 
| Journal of Applied Animal Research | Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an approach to bovine digital dermatitis[10.1080:09712119.2017.1335208] | 
| PLoS One | Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis[Pubmed: 30939166] | 
| МАТРИКСНЫЕ МЕТАЛЛОПРОТЕИНАЗЫ: ЗНАЧЕНИЕ В РЕМОДЕЛИРОВАНИИ СЕРДЦА У ПАЦИЕНТОВ С ДИСПЛАЗИЕЙ СОЕДИНИТЕЛЬНОЙ ТКАНИ[] | |
| International Journal of Molecular Sciences | A New Analytical Method for Determination of Cathepsin L Based on the Surface Plasmon Resonance Imaging Biosensor[Pubmed: 31052424] | 
| INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY | MT 1‐MMP evaluation in neointimal hyperplasia in the late follow‐up after prosthesis implantation[] | 
| Journal of Plastic, Reconstructive & Aesthetic Surgery | Intraluminal fluid infusion in a rat jejunum ischemia/reperfusion model is associated with improved tissue perfusion and less mucosal damage[Pubmed: 31734236] | 
| Bone Research | Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis[Pubmed: 32047705] | 
| Scientific Reports | Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis[Pubmed: 31980722] | 
| JOURNAL OF ETHNOPHARMACOLOGY | Danlou tablet inhibits the inflammatory reaction of high-fat diet-induced atherosclerosis in ApoE knockout mice with myocardial ischemia via the NF-κB signaling …[Pubmed: 32745509] | 
| Food Funct | Undenatured type II collagen prevents and treats the osteoarthritis and motor function degradation in T2DM patients and db/db mice[33890588] | 
| Phytomedicine | Transcriptomic profiling revealed the role of apigenin-4′-O-α-L-rhamnoside in inhibiting the activation of rheumatoid arthritis fibroblast-like synoviocytes via MAPK …[Pubmed:35660352] | 
| Journal of Immunology Research | Ebosin Attenuates the Inflammatory Responses Induced by TNF-α through Inhibiting NF-κB and MAPK Pathways in Rat Fibroblast-Like Synoviocytes[Pubmed:35340587] | 




