摄食抑制因子1(NES1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Nesfatin 1 (NES1)
- 编号CEA242Mu
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围493.8-40,000pg/mL
- 灵敏度最小可检测剂量小于等于183.4pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载英文说明书 中文说明书
- 规格48T96T96T*596T*1096T*100
- 价格¥ 2722¥ 3888¥ 17496¥ 33048¥ 272160
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
产品包装(模拟)
产品包装(模拟)
实验结果图
标准曲线图
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测摄食抑制因子1(NES1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的摄食抑制因子1(NES1)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 80-101 | 86 |
| EDTA plasma(n=5) | 96-104 | 101 |
| heparin plasma(n=5) | 87-96 | 90 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的摄食抑制因子1(NES1)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中摄食抑制因子1(NES1)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-98% | 80-90% | 96-103% | 83-101% |
| EDTA plasma(n=5) | 83-90% | 96-103% | 97-105% | 84-103% |
| heparin plasma(n=5) | 78-94% | 82-89% | 80-101% | 87-96% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分钟；
7. 加终止液50µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将摄食抑制因子1(NES1)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA242Mu01 | 摄食抑制因子1(NES1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA242Mu01 | 摄食抑制因子1(NES1)活性蛋白 | Cell culture; Activity Assays. |
| PAA242Mu01 | 摄食抑制因子1(NES1)多克隆抗体 | WB; IHC; ICC; IP. |
| SEA242Mu | 摄食抑制因子1(NES1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA242Mu | 摄食抑制因子1(NES1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA242Mu | 摄食抑制因子1(NES1)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA242Mu | 摄食抑制因子1(NES1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Bulletin of Clinical Psychopharmacology | Plasma Nesfatin-1 Level May Be Associated with Disease Severity in Patients with Panic Disorder[Psikofarmakoloji: 2043] |
| Progress in Neuro-Psychopharmacology & Biological Psychiatry | High plasma nesfatin-1 level in patients with major depressive disorder[PubMed: 21163317] |
| Endocrine | Cord blood nesfatin-1 and apelin-36 levels in gestational diabetes mellitus[SpringerLink: h978088657555w16] |
| Bulletin of Clinical Psychopharmacology | Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsive Disorder [Psikofarmakoloji: 2212] |
| Psychoneuroendocrinology. | Decreased plasma nesfatin-1 levels in patients with generalized anxiety disorder[PubMed: 22595767] |
| Iranian Journal of Health and Physical activity | Effects of Aerobic Training, with or without Zizyphus Jujuba Water Extraction, on Fundus Nesfatin-1, ATP, HDL-C, and LDL-C Concentrations in Female Rats[Ijvst: Source] |
| European Journal of Obstetrics & Gynecology and Reproductive Biology | Decreased serum nesfatin-1 levels in endometriosis[Pubmed: 24702902] |
| Bulletin of Clinical Psychopharmacology | Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsie Disorder.[Psikofarmakoloji: Source] |
| Current Pharmaceutical Design | Role of NUCB2/nesfatin-1 as a possible biomarker.[Pubmed: 23537082] |
| Regulatory, Integrative and Comparative Physiology | A role of nesfatin-1/NucB2 in dehydration-induced anorexia[Pubmed:24829503] |
| J Endocrinol Invest. | Plasma nesfatin-1 levels are increased in patients with polycystic ovary syndrome[Pubmed:24920281] |
| Int J Surg. | The potential role of BMI, plasma leptin, nesfatin-1 and ghrelin levels in the early detection of pancreatic necrosis and severe acute pancreatitis: a prospective cohort study.[Pubmed:25448651] |
| Hormone molecular biology and clinical investigation | The effects of short-term high-intensity interval training vs. moderate-intensity continuous training on plasma levels of nesfatin-1 and inflammatory markers[Pubmed:25581765] |
| Turk J Gastroenterol. | New hormones to predict the severity of gallstone-induced acute pancreatitis[Pubmed:25599787] |
| Respiration | Is There a Relationship between Obstructive Sleep Apnea Syndrome Severity and Nesfatin-1?[PubMed: 26088934] |
| J Clin Res Pediatr Endocninol | Correlation of Brain Neuropeptide (Nesfatin-1 and Orexin-A) Concentrations with Anthropometric and Biochemical Parameters in Malnourished Children[PubMed: 26831553] |
| Bosn J Basic Med Sci | Nesfatin-1 and Vitamin D levels may be associated with systolic and diastolic blood pressure values and hearth rate in polycystic ovary syndrome[PubMed: 26295295] |
| Original Article / Orijinal Makale | Relationship and Interaction between Anaerobic Sports Branches and Serum Nesfatin-1[Sayilar: 294] |
| Gynecol Endocrinol | Nesfatin-1 levels and metabolic markers in polycystic ovary syndrome[PubMed: 26062107] |
| International Journal of Neuroscience | Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage*[Pubmed:26872406] |
| 未找到 | Comparing the nesfatin-1 levels between individuals with subjective cognitive decline and mild cognitive impairment[P.1.e.012.pdf] |
| International Journal of Developmental | Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage.[pubmed:26872406] |
| Current Pharmaceutical Design | Metabolic and Cardiovascular Actions of Nesfatin-1: Implications in Health and Disease[pubmed:28137218] |
| Arch Endocrinol Metab | Serum nesfatin-1 levels are decreased in pregnant women newly diagnosed with gestational diabetes[pubmed:28977160] |
| Indian Journal of Psychiatry | Serum NUCB2/nesfatin-1 levels in different stages of alcohol dependence: Is there a relationship with craving?[pubmed:28529367] |
| Biomedicine & Pharmacotherapy | Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ[Pubmed:29604590] |
| Biochimica et Biophysica Acta | Nesfatin-1 functions as a switch for phenotype transformation and proliferation of VSMCs in hypertensive vascular remodeling[Pubmed:29627363] |
| BMC Psychiatry | Plasma nesfatin-1 level is associated with severity of depression in Chinese depressive patients[Pubmed:29615007] |
| Probiotics and Antimicrobial Proteins | Mitra Zarrati, Mahsa Raji Lahiji, Eisa Salehi, Bahareh Yazdani, Elham Razmpoosh, Raheleh Shokouhi Shoormasti & Farzad Shidfar[Pubmed: 30232744] |
| An elevated serum level of nesfatin-1 may predict poor outcomes in patients with aneurysmal subarachnoid hemorrhages[] | |
| clinical neurology and neurosurgery | Relationshıp of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levels[Pubmed: 32311618] |
| Ann Nutr Metab | Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity[Pubmed: 33027789] |
| Endokrynologia Polska | Assessing the blood concentration of new adipocytokines in patients with ischaemic stroke[33283259] |
| Increased serum nesfatin-1 levels among adolescents diagnosed with major depressive disorder[] |
