武汉云克隆科技股份有限公司

摄食抑制因子1(NES1)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Nesfatin 1 (NES1)

    • 摄食抑制因子1(NES1)检测试剂盒(酶联免疫吸附试验法)产品包装(模拟)
    • 摄食抑制因子1(NES1)检测试剂盒(酶联免疫吸附试验法)产品包装(模拟)
    • 摄食抑制因子1(NES1)检测试剂盒(酶联免疫吸附试验法)实验结果图
    • Certificate通过ISO 9001、ISO 13485质量体系认证

    特异性

    本试剂盒用于检测摄食抑制因子1(NES1)，经检测与其它相似物质无明显交叉反应。
    由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

    回收率

    分别于定值血清及血浆样本中加入一定量的摄食抑制因子1(NES1)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

    样本回收率范围(%)平均回收率(%)
    serum(n=5)85-9289
    EDTA plasma(n=5)79-10185
    heparin plasma(n=5)97-104101

    精密度

    精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
    批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
    批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
    批内差： CV<10%
    批间差： CV<12%

    线性

    在定值血清及血浆样本内加入适量的摄食抑制因子1(NES1)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中摄食抑制因子1(NES1)含量的测定值与理论值的比率。

    样本1:21:41:81:16
    serum(n=5)79-99%79-93%92-101%79-104%
    EDTA plasma(n=5)91-99%91-105%89-103%78-95%
    heparin plasma(n=5)83-101%98-105%78-95%99-105%

    稳定性

    经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
    为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

    实验流程

    1. 实验前标准品、试剂及样本的准备；
    2. 加样（标准品及样本）100µL，37°C孵育1小时；
    3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
    4. 洗板3次；
    5. 加检测溶液B100µL，37°C孵育30分钟；
    6. 洗板5次；
    7. 加TMB底物90µL，37°C孵育10-20分钟；
    8. 加终止液50µL，立即450nm读数。

    实验原理

    将摄食抑制因子1(NES1)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的摄食抑制因子1(NES1)与连接于固相载体上的抗体结合，然后加入生物素化的摄食抑制因子1(NES1)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的摄食抑制因子1(NES1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

    相关产品

    编号适用物种：Mus musculus (Mouse，小鼠)应用(仅供研究使用，不用于临床诊断！)
    RPA242Mu01摄食抑制因子1(NES1)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
    APA242Mu01摄食抑制因子1(NES1)活性蛋白Cell culture; Activity Assays.
    PAA242Mu01摄食抑制因子1(NES1)多克隆抗体WB; IHC
    SEA242Mu摄食抑制因子1(NES1)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
    CEA242Mu摄食抑制因子1(NES1)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
    MEA242Mu摄食抑制因子1(NES1)检测试剂盒(酶联免疫吸附试验法,小样本)Enzyme-linked immunosorbent assay for Antigen Detection.
    LMA242Mu摄食抑制因子1(NES1)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.

    参考文献

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