血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Angiotensin I Converting Enzyme (ACE) ,etc. by FLIA (Flow Luminescence Immunoassay)
CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form
（注：单次混测多因子不超过8个指标 ）
- 编号LMA004Po
- 物种Sus scrofa; Porcine (Pig，猪)相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围1.95-2000pg/mL
- 灵敏度最小可检测剂量小于等于0.65 pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载英文说明书 中文说明书
- 规格8指标数7指标数6指标数5指标数4指标数3指标数2指标数1指标数
- 价格¥ 2830¥ 2939¥ 3102¥ 3320¥ 3537¥ 3864¥ 4354¥ 5442 添加到价格计算器 计算器
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
特异性
本试剂盒用于检测血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 86-101 | 91 |
| EDTA plasma(n=5) | 89-101 | 94 |
| heparin plasma(n=5) | 95-104 | 98 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 92-101% | 84-99% | 82-103% | 97-104% |
| EDTA plasma(n=5) | 98-105% | 80-99% | 89-104% | 86-99% |
| heparin plasma(n=5) | 88-95% | 98-105% | 91-105% | 95-104% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Sus scrofa; Porcine (Pig，猪) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA004Po01 | 血管紧张素转化酶(ACE)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA004Po01 | 血管紧张素转化酶(ACE)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA004Po81 | 血管紧张素转化酶(ACE)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAA004Po71 | 血管紧张素转化酶(ACE)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA004Po21 | 血管紧张素转化酶(ACE)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA004Po | 血管紧张素转化酶(ACE)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA004Po | 血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Lipids in Health and disease | Association of elevated blood pressure and impaired vasorelaxation in experimental Sprague-Dawley rats fed with heated vegetable oil[PubMed: PMC2914008] |
| Malaysian Journal of Medical Sciences | Effect of Repeatedly Heated Palm Olein on Blood Pressure–Regulating Enzymes Activity and Lipid Peroxidation in Rats[Usm: MJMS19120] |
| Journal of Cancer Therapy | Plasma Levels of Angiotensin-Converting Enzymes 1 and 2 and AGTR2 (T1247G and A5235G) Gene Polymorphisms Are Associated to Breast Cancer Progression.[Scirp: Source] |
| The American journal of clinical nutrition | Vascular function and atherosclerosis progression after 1 y of flavonoid intake in statin-treated postmenopausal women with type 2 diabetes: a double-blind randomized controlled trial.[Pubmed: 23553151] |
| Journal of Vascular Surgery | Cathepsin G deficiency reduces periaortic calcium chloride injury-induced abdominal aortic aneurysms in mice [Pubmed:25037606] |
| Reprod Toxicol.? | ACE2 activation by xanthenone prevents leptin-induced increases in blood pressure and proteinuria during pregnancy in Sprague-Dawley rats[Pubmed:25205467] |
| Chemical Research in Toxicology | Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation[Pubmed:25580880] |
| Chem Biol Interact | Arsenic causes aortic dysfunction and systemic hypertension in rats: Augmentation of angiotensin II signaling[PubMed: 26079204] |
| Biomed Res Int | Protective Effect of Enalapril against Methionine-Enriched Diet-Induced Hypertension: Role of Endoplasmic Reticulum and Oxidative Stress[PubMed: 26640794] |
| Clinics (Sao Paulo) | Mechanisms[PubMed: 26602523] |
| Chemical Research in Toxicology | Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation[PubMed: 25580880] |
| Intensive Care Medicine Experimental | Angiotensin converting enzymes in patients with acute respiratory distress syndrome[Content: 3] |
| Evid Based Complement Alternat Med. | Garlic Attenuates Plasma and Kidney ACE-1 and AngII Modulations in Early Streptozotocin-Induced Diabetic Rats: Renal Clearance and Blood Pressure Implications[Pubmed:27293465] |
| Вавиловский журнал генетики и селекции | Ренин-ангиотензин-альдостероновая система у крыс линии НИСАГ (ISIAH) со стресс-индуцированной артериальной гипертензией[30_Dubinina.pdf] |
| Animal Biology | The effect of heat stress on bull sperm quality and related HSPs expression[content:journals] |
| biomedicine & pharmacotherapy | Citrus leaf extract reduces blood pressure and vascular damage in repeatedly heated palm oil diet-Induced hypertensive rats.[pubmed:28068636] |
| Biomedicine & Pharmacotherapy | Parkia speciosa empty pod prevents hypertension and cardiac damage in rats given N(G)-nitro- L -arginine methyl ester[pubmed:28992471] |
| Journal of Functional Foods | Antihypertensive effects of Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation[10.1016/j.jff.2017.05.033] |
| Molecules | Protective Effect of Polyphenol-Rich Extract from Bee Pollen in a High-Fat Diet[Pubmed:29614743] |
| Journal of Surgical Research | Impact of angiotensin-converting enzyme 2 levels on postoperative pneumonia after esophagectomy[Pubmed:29506841] |
| Intensive Care Medicine | Angiotensin-converting enzymes in acute respiratory distress syndrome[] |
| International Journal of Biological Sciences | Vascular Protection of TPE-CA on Hyperhomocysteinemia-induced Vascular Endothelial Dysfunction Through AA Metabolism Modulated CYPs Pathway[] |
| Medicine | Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study[Pubmed: 32756181] |
| American Journal of Respiratory and Critical Care Medicine | Equilibrium Angiotensin Metabolite Profiling in Patients with Acute Respiratory Distress Syndrome Indicates Angiotensin Converting Enzyme Inhibition[Pubmed: 32628511] |
| Int Immunopharmacol | Crocin protects against cardiotoxicity induced by doxorubicin through TLR-2/NF-κB signal pathway in vivo and vitro[Pubmed: 32388215] |
| Food Bioscience | Djulis (Chenopodium formosanum) and its bioactive compounds affect vasodilation, angiotensin converting enzyme activity, and hypertension[] |
| Journal of Extracellular Vesicles | MiR155-5p in adventitial fibroblasts-derived extracellular vesicles inhibits vascular smooth muscle cell proliferation via suppressing angiotensin-converting enzyme …[] |
| Biotechnology and Pharmacy | Chrysin pretreatment improves mitochondrial enzymes and angiotensin converting enzymes in L-NAME induced hypertensive rats[] |
| Dikkat Eksikliği Hiperaktivite Bozukluğu Tanısı Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin Dönüştürücü Enzim (ACE), NADPH Oksidaz Enzim Serum …[] |