武汉云克隆科技股份有限公司

膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Annexin A2 (ANXA2) ,etc. by FLIA (Flow Luminescence Immunoassay)

ANX-A2; ANX2L4; CAL1H; LIP2; LPC2; LPC2D; P36; PAP-IV; Protein I; Chromobindin-8; Calpactin-1 heavy chain; Lipocortin II; Placental anticoagulant protein IV

（注：单次混测多因子不超过8个指标 ）

  • 膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)产品包装(模拟)
  • 膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)产品包装(模拟)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本回收率范围(%)平均回收率(%)
serum(n=5)80-9588
EDTA plasma(n=5)85-9994
heparin plasma(n=5)84-10193
sodium citrate plasma(n=5)94-10197

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本1:21:41:81:16
serum(n=5)91-102%98-105%80-90%80-101%
EDTA plasma(n=5)80-101%78-96%97-104%79-96%
heparin plasma(n=5)83-99%86-95%82-92%96-104%
sodium citrate plasma(n=5)80-92%89-103%93-105%90-98%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
    37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号适用物种：Homo sapiens (Human，人)应用(仅供研究使用，不用于临床诊断！)
RPB944Hu03膜联蛋白A2(ANXA2)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPB944Hu01膜联蛋白A2(ANXA2)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPB944Hu04膜联蛋白A2(ANXA2)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APB944Hu01膜联蛋白A2(ANXA2)活性蛋白Cell culture; Activity Assays.
RPB944Hu02膜联蛋白A2(ANXA2)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAB944Hu01膜联蛋白A2(ANXA2)多克隆抗体WB; IHC; ICC; IP.
PAB944Hu02膜联蛋白A2(ANXA2)多克隆抗体WB; IHC; FCM
LAB944Hu71膜联蛋白A2(ANXA2)多克隆抗体(生物素标记)WB; IHC; ICC.
MAB944Hu22膜联蛋白A2(ANXA2)单克隆抗体WB; IHC; ICC; IP.
MAB944Hu21膜联蛋白A2(ANXA2)单克隆抗体WB; IHC; ICC; IP.
FAB944Hu08抗膜联蛋白A2(ANXA2)单克隆抗体Flow cytometry.
FAB944Hu06抗膜联蛋白A2(ANXA2)单克隆抗体Flow cytometry.
FAB944Hu04抗膜联蛋白A2(ANXA2)单克隆抗体Flow cytometry.
FAB944Hu02抗膜联蛋白A2(ANXA2)单克隆抗体Flow cytometry.
SEB944Hu膜联蛋白A2(ANXA2)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
SCB944Hu膜联蛋白A2(ANXA2)检测试剂盒(化学发光免疫分析法)Chemiluminescent immunoassay for Antigen Detection.
LMB944Hu膜联蛋白A2(ANXA2)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.

参考文献

杂志参考文献
Journal of Cellular BiochemistrySubcellular proteome analysis unraveled annexin A2 related to immune liver fibrosis[PubMed: 20225235]
World Journal of GastroenterologyExpression characteristics and diagnostic value of annexin A2 in hepatocellular carcinoma[PubMed: PMC3491596]
Oncology LettersAnnexin A2 is not a good biomarker for hepatocellular carcinoma in cirrhosis[PubMed: PMC3742823]
IbrahimAnnexin A2 Versus Afp as An Efficient Diagnostic Serum Marker for Hepatocellular Carcinoma[Ghrnet: Source]
European Journal of Clinical Microbiology &amp; Infectious DiseasesSerum annexin A2 levels in acute brucellosis and brucellar spondylodiscitis[Pubmed:24853056]
Biomedicine &amp; PharmacotherapyCirculating annexin A2 as a biomarker in gastric cancer patients: Correlation with clinical variables[Pubmed:25661364]
Hepat MonSerum Levels of Annexin A2 as a Candidate Biomarker for Hepatic Fibrosis in Patients With Chronic Hepatitis B[PubMed: 26587036]
Tumour BiolUp-regulation of annexin A2 expression predicates advanced clinicopathological features and poor prognosis in hepatocellular carcinoma[PubMed: 26109000]
Biomed PharmacotherCirculating[PubMed: 25661364]
Tumour BiolEvaluation of annexin A2 and as potential biomarkers for hepatocellular carcinoma[PubMed: 26189841]
Eur J Clin InvestStairs instead of elevators at the workplace decreases PCSK9 levels in a healthy population[PubMed: 26081791]
Journal of Oral and Maxillofacial SurgerySerum Annexin A2 Level Is Associated With Diagnosis and Prognosis in Patients With OralSquamous Cell Carcinoma.[pubmed:27889534]
Pregnancy HypertensAssessment of annexin A5 and annexin A2 levels as biomarkers for pre-eclampsia: A pilot study.[pubmed:28501283]
Mol Nutr Food Res.Epigallocatechin gallate induces an up-regulation of LDL receptor accompanied by a reduction of PCSK9 via the annexin A2-independent pathway in HepG2 cells.[pubmed:28181408]
Archives of Gynecology and ObstetricsExpression of annexin A2 in adenomyosis and dysmenorrhea[Pubmed: 31183557]
CancersExtracellular Vesicles-Based Biomarkers Represent a Promising Liquid Biopsy in Endometrial Cancer[Pubmed: 31842290]
PLASMA BIOMARKER FOR OVARIAN CANCER[]
DiagnosticsDiagnostic Value of Plasma Annexin A2 in Early-Stage High-Grade Serous Ovarian Cancer. Diagnostics 2021, 11, 69[33406648]
ADVANCED RESEARCHESSearch for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C[]
Cancer Cell InternationalBufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins[]
Cancer Cell IntBufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression[34376212]