武汉云克隆科技股份有限公司

Abstract：

OBJECTIVE：

Elevated lipopolysaccharide-binding protein (LBP), a marker of subclinical endotoxemia, may be involved in the pathogenesis of obesity and metabolic risk. We aimed to investigate the association between plasma LBP and metabolic disorders in apparently healthy Chinese.

RESEARCH DESIGN AND METHODS：

A population-based study including 559 overweight/obese (BMI ≥24.0 kg/m2) and 500 normal-weight (18.0 ≤ BMI <24.0 kg/m2) subjects aged 35–54 years was conducted in Shanghai, China. Fasting plasma glucose, lipid profile, LBP, high-sensitivity C-reactive protein, interleukin-6, high-molecular-weight (HMW) adiponectin, leptin, hepatic enzymes, and body composition were measured. Metabolic syndrome was defined by the updated National Cholesterol Education Program Adult Treatment Panel III criterion for Asian Americans.

RESULTS：

LBP levels were significantly higher in overweight/obese individuals than in normal-weight individuals (geometric mean 27.6 [95% CI 25.2–30.3] vs. 10.0 [9.1–11.1] μg/ml; P< 0.001). After multiple adjustments including BMI, the odds ratios were 3.54 (95% CI 2.05–6.09) and 5.53 (95% CI 2.64–11.59) for metabolic syndrome and type 2 diabetes, respectively, comparing the highest with the lowest LBP quartile. Further adjustments for inflammatory markers almost abolished the significant association of LBP with metabolic syndrome but not that with type 2 diabetes, and controlling for adipokines and hepatic enzymes did not substantially alter the results.

CONCLUSIONS：

Elevated circulating LBP was associated with obesity, metabolic syndrome, and type 2 diabetes in apparently healthy Chinese. These findings suggested a role of lipopolysaccharide via initiation of innate immune mechanism(s) in metabolic disorders. Prospective studies are needed to confirm these results.

摘要：

背景：

脂多糖结合蛋白（LBP）是亚临床内毒素血症的一种标记物，可能参与了肥胖和代谢风险的发病机制。本研究旨在探讨中国表观健康人群血浆LBP水平与代谢紊乱的关系。

方法：

在中国上海进行了一项基于人群的研究，包括559名超重/肥胖者（BMI ≥24.0 kg/m2）和500名体重正常者（18.0 ≤ BMI <24.0 kg/m2），受试者年龄范围为35–54岁。对每一位受试者测定空腹血糖、血脂、LBP、高敏C-反应蛋白、白细胞介素-6、高分子量脂联素、瘦素、肝脏酶和体躯成分。代谢综合征由最新的美国国家胆固醇教育计划成人治疗组第三次指南(ATPⅢ)来定义。

结果：

超重/肥胖人群的血浆LBP水平明显高于体重正常者（几何平均数为27.6[95%CI 25.2~30.3]和10.0[9.1~11.1]ug/ml；P<0.001）。包括BMI在内的多次调整后，比较最高与最低LBP四位分值，代谢综合征和2型糖尿病比率3.54（95%CI 2.05-6.09）和5.53（95%CI 2.64-11.59）。炎症标志物的进一步调节几乎消除了LBP与代谢综合征的显著相关性，但2型糖尿病依然与LBP相关。同时LBP对脂肪因子和肝脏酶的控制并不会对结果造成很大的影响。

结论：

循环LBP的增加与中国表观健康人群肥胖、代谢综合征和2型糖尿病有关。这些发现揭示脂多糖通过启动先天免疫机制在代谢紊乱中发挥作用。需要前瞻性研究来证实这些结果。

使用试剂原文信息：Plasma LBP levels were determined by a sandwich ELISA (USCN Life Science & Technology, Missouri City, TX).