武汉云克隆科技股份有限公司

Abstract: Blood vessels in the mammalian skeletal system control bone formation and support haematopoiesis by generating local niche environments. While a specialized capillary subtype, termed type H, has been recently shown to couple angiogenesis and osteogenesis in adolescent, adult and ageing mice, little is known about the formation of specific endothelial cell populations during early developmental endochondral bone formation. Here, we report that embryonic and early postnatal long bone contains a specialized endothelial cell subtype, termed type E, which strongly supports osteoblast lineage cells and later gives rise to other endothelial cell subpopulations. The differentiation and functional properties of bone endothelial cells require cell–matrix signalling interactions. Loss of endothelial integrin β1 leads to endothelial cell differentiation defects and impaired postnatal bone growth, which is, in part, phenocopied by endothelial cell-specific laminin α5 mutants. Our work outlines fundamental principles of vessel formation and endothelial cell differentiation in the developing skeletal system.

摘要：在哺乳动物骨骼系统中，血管通过生成局部环境来控制骨骼的形成和支持造血功能。最近的研究表明，在青少年、成年和年老的小鼠中，一种被称为H型的特殊毛细管亚型可以连合血管生成和骨生成，但人们对在早期发育软骨内的骨形成过程中特定内皮细胞群的形成知之甚少。在此，我们将展示我们的研究成果。胚胎和早期出生的长骨中含有一种专门的内皮细胞亚型，称为E型，它对成骨细胞系细胞有强烈的支持作用，随后产生其他内皮细胞亚群。骨内皮细胞的分化和功能特性需要细胞-基质信号的相互作用。内皮整合素β1的缺失导致了内皮细胞分化缺陷和产后骨骼生长受损，这在一定程度上是由内皮细胞特异性层粘连蛋白α5突变体造成的。我们的工作概述了在骨骼系统发育中血管生成和内皮细胞分化的基本原理。

使用试剂原文信息：Serum levels of parathyroid hormone and calcitonin were measured by quantitative enzyme-linked immunosorbent assay (ELISA) kits (CEA866Mu and CEA472Mu, Cloud-Clone Corp.) in blood samples from mutant and control mice.