武汉云克隆科技股份有限公司

Abstract:

Glycated haemoglobin (HbA1c) is the most important marker of hyperglycaemia in diabetes mellitus. We show that d-ribose reacts with haemoglobin, thus yielding HbA1c. Using mass spectrometry, we detected glycation of haemoglobin with d-ribose produces 10 carboxylmethyllysines (CMLs). The first-order rate constant of fructosamine formation for d-ribose was approximately 60 times higher than that for d-glucose at the initial stage. Zucker Diabetic Fatty (ZDF) rat, a common model for type 2 diabetes mellitus (T2DM), had high levels of d-ribose and HbA1c, accompanied by a decrease of transketolase (TK) in the liver. The administration of benfotiamine, an activator of TK, significantly decreased d-ribose followed by a decline in HbA1c. In clinical investigation, T2DM patients with high HbA1c had a high level of urine d-ribose, though the level of their urine d-glucose was low. That is, d-ribose contributes to HbA1c, which prompts future studies to further explore whether d-ribose plays a role in the pathophysiological mechanism of T2DM.

摘要：

糖化血红蛋白(HbA1c)是糖尿病高血糖的重要标志。研究表明d-核糖与血红蛋白反应产生HbA1c。我们采用质谱法检测D-核糖产生10种羧甲基赖氨酸(CMLs)来测量血红蛋白糖基化。d-核糖生成果糖胺的一级速率常数比初始阶段的d-葡萄糖高约60倍。2型糖尿病的常见模型：Zucker糖尿病大鼠，具有较高水平的d-核糖和HbA1c，肝组织中转酮醇酶(TK)较低。苯磷硫胺是TK的活化剂，能显著降低d-核糖，随之降低HbA1c。在临床调查中，HbA1c高的T2DM患者虽然尿d-葡萄糖水平较低，但尿d-核糖水平较高。这意味着d-核糖对HbA1c有一定的促进作用，今后的研究将进一步探讨d-核糖能否在T2DM的病理机制中发挥作用。

使用试剂原文信息：The levels of HbA1c were detected with an Enzyme-linked Immunosorbent Assay kit (CEA190Hu, Cloud-Clone Corp., China) for human HbA1c each day.