武汉云克隆科技股份有限公司

Abstract: Diabetic skin ulcer is difficult to heal due to the lack of cellular and molecular signals required for normal wound repair. Emulsion electrospinning was adopted to imbed basic fibroblast growth factor (bFGF) into ultrafine fibers with a core-sheath structure to promote the wound healing process. An initially burst release as low as 14.0±2.2% was achieved, followed by gradual release for around 4 wk. In vitro investigations on mouse embryo fibroblasts indicated that bFGF-loaded fibrous mats enhanced cell adhesion, proliferation, and secretion of extracellular matrix (ECM). Skin wounds were created in the dorsal area of diabetic rats for in vivo evaluation of skin regeneration after covered with bFGF-loaded fibrous mats. Compared with fibrous mats infiltrated with free bFGF, bFGF-loaded scaffolds revealed significantly higher wound recovery rate with complete re-epithelialization and regeneration of skin appendages. Higher density and mature capillary vessels were generated during 2 wk after treatment with bFGF-loaded fibers, and there was no fiber fragment observed in the histological sections at week 4 after operation. The gradual release of bFGF from fibrous mats enhanced collagen deposition and ECM remodeling, and the arrangement and component of collagen fibers were similar to normal tissues. The above results demonstrate the potential use of bFGF-loaded electrospun fibrous mats to rapidly restore the structural and functional properties of wounded skin for patients with diabetic mellitus.

摘要：由于缺乏正常伤口修复所需的细胞和分子信号，糖尿病性皮肤溃疡难以愈合。采用乳液静电纺丝法将碱性成纤维细胞生长因子（bFGF）嵌入具有核 - 壳结构的超细纤维中以促进伤口愈合过程。最初爆发释放低至14.0±2.2％，然后逐渐释放约4周。小鼠胚胎成纤维细胞的体外研究表明载有bFGF的纤维垫增强了细胞粘附，增殖和细胞外基质（ECM）的分泌。在糖尿病大鼠的背部区域中创建皮肤伤口，用于体内评估覆盖有bFGF的纤维垫之后的皮肤再生。与用游离碱性成纤维细胞生长因子浸润的纤维垫相比，载有bFGF的支架具有显着更高的伤口恢复率，具有完全的上皮再生和皮肤附属器的再生。在用bFGF加载的纤维处理后2周期间产生更高密度和成熟的毛细血管，并且在术后第4周在组织切片中未观察到纤维碎片。 bFGF从纤维垫逐渐释放，促进胶原沉积和ECM重塑，胶原纤维的排列和成分与正常组织相似。上述结果表明bFGF-负载的电纺纤维垫可能用于快速恢复患有糖尿病的患者的创伤皮肤的结构和功能特性。

使用试剂原文信息：Core loading of bFGF in electrospun fibers was determined by an ELISA kit (Uscn Life Science Inc., Wuhan, China) based on the manufacture’s protocol afterextracting from fiber samples.