锶和唑来膦酸对羟基磷灰石结构和骨细胞反应的联合作用
文献 Combined effect of strontium and zoledronate on hydroxyapatite structure and bone cell responses 于 2014年 发表在 BIOMATERIALS 原文链接
Abstract: The influence of the simultaneous presence of the two inhibitors of bone degradation, strontium and zoledronate, on the direct synthesis of hydroxyapatite was explored in the range of Sr concentration up to 50 atom% at two different bisphosphonate concentrations (ZOL7 and ZOL14). The results of structural analysis indicated that HA can be obtained as a single crystalline phase up to a Sr concentration in solution of 20 and 10 atom% within the ZOL7 and ZOL14 series respectively. Both Sr substitution and ZOL incorporation affect the length of the coherently scattering crystalline domains and the dimensions of HA nanocrystals. At greater Sr content, XRD full profile fitting data indicate that zoledronate provokes the segregation of Sr in two crystalline apatitic phases, at different strontium content. Co-cultures of osteoblast-like MG63 cells and human osteoclast show that ZOL displays a greater inhibitory influence than Sr on osteoclast proliferation and activity. On the other hand, the results obtained on osteoblast surnatant and on gene expression indicate that Sr exerts a greater promotion on osteoblast proliferation and differentiation. The co-presence of Sr and ZOL has a combined effect on the differentiation markers, so that HA containing about 4 wt% ZOL and 4 Sr atom%, and even more HA containing about 4 wt% ZOL and 8 Sr atom%, result the best compromise for osteoblast promotion and osteoclast inhibition.
摘要：在两种不同的双膦酸盐浓度（ZOL7和ZOL14）下，在Sr浓度高达50原子％的范围内探索了同时存在两种骨降解抑制剂锶和唑来膦酸对羟基磷灰石的直接合成的影响。结构分析的结果表明，HA可以以ZOL7和ZOL14系列中分别为20和10原子％的溶液中的Sr浓度达到单晶相的形式获得。Sr替代和ZOL掺入都影响相干散射结晶域的长度和HA纳米晶体的尺寸。在Sr含量较高时，XRD全剖面拟合数据表明，在不同的锶含量下，zoledronate激发两种结晶磷灰石相的Sr分离。成骨细胞样MG63细胞和人破骨细胞的共培养显示ZOL比Sr对破骨细胞增殖和活性具有更大的抑制作用。另一方面，成骨细胞上清液和基因表达的结果表明Sr对成骨细胞增殖和分化有更大的促进作用。 Sr和ZOL的共存对分化标记物具有综合作用，使得含有约4wt％ZOL和4 Sr原子％的HA，甚至更多HA含有约4wt％ZOL和8 Sr原子％的HA导致成骨细胞促进和破骨细胞抑制的最佳比例。
使用试剂原文信息：Aliquots were dispensed in Eppendorf tubes for storage at -70℃ and assayed for Alkaline Phosphatase (ALP, immunoenzymatic assay, Immunodiagnostics, UK), Type I Pro-Collagen (Coll1, immunoassay kit, USCN Life Science, Wuhan, China), Osteocalcin (OSTC immunoassay kit, USCN Life Science)