武汉云克隆科技股份有限公司

Abstract:

Background:

Squamous cell carcinoma antigen (SCC-Ag) is a serologic tumor marker detected in SCC of the cervix. The aim of this study is clinical features and their histopathological correlation between preinvasive and invasive cervical cancer and its association with SCC-Ag levels.

Materials and Methods:

This case–control study was carried out over a period of 1 year in the Department of Obstetrics and Gynaecology, with collaboration of pathology and medicine. After informed consent and ethical clearance, totally 3200 women were recruited. Out of these, 76 women who were histopathological proven, 30 preinvasive, and 46 of invasive cervical malignancy (International Federation of Gynecology and Obstetrics Stage I–IV) enrolled for study. 15 healthy cytology negative were considered as controls. Per speculum, per vaginam examination was done in every women and pap smear was obtained. Pretreatment 5 ml venous blood samples were drawn into sterile vials. SCC-Ag levels were measured by enzyme-linked immunosorbent assay (ELISA) technique using ELISA Kit as per producer protocol.

Results:

Among preinvasive group, 35.46% women complained white discharge per vaginam. Blood mixed discharge and postcoital bleeding were observed in 3.4% and 0.71%, respectively. In malignant group, foul smelling discharge and postmenopausal bleeding were reported in 1.68% and 1.87% women, respectively. Serum SCC-Ag levels were increased from controls to cases. In controls, 0.27 ± 0.12 ng/ml, preinvasive 0.85 ± 0.37 ng/ml and in invasive malignancy Stage I, II, III, IV, 2.10 ± 0.55 ng/ml, 3.15 ± 0.84 ng/ml, 4.12 ± 0.89 ng/ml, and 2.71 ± 1.05 ng/ml, respectively. Moderately differentiated and poorly differentiated SCC were reported in 80.43% and 19.56%, respectively. Expired patients had significantly (P < 0.01) higher premean SCC Ag level as compared to those who remain alive.

Conclusion:

Serum SCC-Ag is not only useful in the detection of preinvasive lesions and early invasive cases of cervical cancer but also a definite indicator for advanced Stage malignancy. Its value was quite high in late stages of cervical malignancy, thus it can be used as reproducible marker in cervical cancer.

摘要：

背景：

鳞状上皮细胞癌抗原（SCC-Ag）是在宫颈鳞状细胞癌中检测到的一种血清肿瘤标记物。本研究旨在探讨浸润性宫颈癌的临床特征、浸润前宫颈癌与浸润性宫颈癌的组织病理学相关性及其SCC-Ag水平的关系。

方法：

这项为期一年的病例对照研究是在妇产科与病理学和医学的合作下进行的。经知情同意和道德审查后，共招募了3200名女性，其中76名女性经病理检查证实30名为浸润前宫颈癌，46名为浸润性宫颈癌（国际妇产科学联合会I–IV期）。以15例健康阴性者作为对照，对每名女性均行一次性阴道镜检，并作巴氏涂片。预处理后取静脉血5ml制成无菌小瓶，并采用酶联免疫吸附法(ELISA)检测SCC-Ag水平。

结果：

在浸润前宫颈癌组中，35.46%例女性阴道分泌物呈白色。血混合排出和产后出血的发生率分别为3.4%和0.71%。在浸润性宫颈癌组中，出现恶臭分泌物和绝经后出血的概率分别为1.68%和1.87%。正常对照组和病例组血清SCC-Ag水平均有升高，对照组中SCC-Ag水平为0.27 ± 0.12 ng/ml；浸润前宫颈癌组中0.85 ± 0.37 ng/ml；在浸润性宫颈癌组中侵袭性恶性阶段I、II、III、IV，SCC-Ag水平分别为2.10 ± 0.55 ng/ml, 3.15 ± 0.84 ng/ml, 4.12 ± 0.89 ng/ml, and 2.71 ± 1.05 ng/ml。中分化和低分化SCC分别占80.43%和19.56%。晚期患者SCC Ag水平明显高于存活者。

结论：

血清SCC-Ag水平不仅有助于宫颈癌侵袭前病变和早期宫颈癌的诊断，也是诊断晚期恶性肿瘤的明确指标。在晚期宫颈癌中具有较高的价值，可作为宫颈癌的可重复标记物。

使用试剂原文信息：Serum SCCAg levels (SCC-Ag2) were estimated by sandwich ELISA technique using ELISA kit forin vitro quantitative measurement as per producer protocol (USCN life sciences Inc., Export Processing zone, Economic and Technological development zone, China).