吲哚胺2,3-双加氧酶(IDO)活性蛋白

Active Indoleamine-2,3-Dioxygenase (IDO)

CD107B; INDO; Indoleamine-Pyrrole 2,3 Dioxygenase

活性实验

IDO (Indoleamine 2,3-dioxygenase 1) is a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. Thus, bioactivity of recombinant human IDO was measured through its ability to oxidize L-tryptophan to N-formyl-kynurenine, using Methylene Blue as indicator. The reaction was performed in 50 mM MES, pH 6.5 (Assay Buffer), initiated by addition 50 μL of various concentrations of IDO (diluted by Assay Buffer) to 50 µL substrate mixture of 800 μM L-tryptophan, 9000 units/mL catalase (RPC418Hu05), and 40 μM Methyene Blue in assay buffer with equal volume of 80 mM ascorbic acid in 0.405 M Tris, pH 8.0. The final well serves as a negative control with no IDO, replaced with 50μL assay buffer. The absorbance was read in 321 nm in kinetic mode for 5 minutes. The result indicated that recombinant human IDO can oxidize L-tryptophan, the specific activity is 10581 pmol/min/μg.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号适用物种:Homo sapiens (Human,人)应用(仅供研究使用,不用于临床诊断!)
RPB547Hu01吲哚胺2,3-双加氧酶(IDO)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APB547Hu01吲哚胺2,3-双加氧酶(IDO)活性蛋白Cell culture; Activity Assays.
PAB547Hu01吲哚胺2,3-双加氧酶(IDO)多克隆抗体WB; IHC; ICC/IF
LAB547Hu71吲哚胺2,3-双加氧酶(IDO)多克隆抗体(生物素标记)WB
LAB547Hu81吲哚胺2,3-双加氧酶(IDO)多克隆抗体(异硫氰酸荧光素标记)WB; IHC; ICC; IF.
MAB547Hu22吲哚胺2,3-双加氧酶(IDO)单克隆抗体WB;ICC/IF;FCM
MAB547Hu24吲哚胺2,3-双加氧酶(IDO)单克隆抗体WB; IHC; ICC; IP.
MAB547Hu21吲哚胺2,3-双加氧酶(IDO)单克隆抗体WB; IHC
MAB547Hu23吲哚胺2,3-双加氧酶(IDO)单克隆抗体WB; IHC; ICC; IP.
FAB547Hu08抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体Flow cytometry.
FAB547Hu06抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体Flow cytometry.
FAB547Hu04抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体Flow cytometry.
FAB547Hu02抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体Flow cytometry.
LAB547Hu72吲哚胺2,3-双加氧酶(IDO)单克隆抗体(生物素标记)WB; IHC; ICC.
SEB547Hu吲哚胺2,3-双加氧酶(IDO)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMB547Hu吲哚胺2,3-双加氧酶(IDO)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
PSB547Hu01吲哚胺2,3-双加氧酶(IDO)抗体对ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSB547Hu01吲哚胺2,3-双加氧酶(IDO)检测试剂盒DIY材料(酶联免疫吸附试验法)Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志参考文献
Blood CCL18 differentiates dendritic cells in tolerogenic cells able to prime regulatory T cells in healthy subjects[Pubmed: 21803856]
Clinical TransplantationPotential immunosuppressive function of plasma indoleamine 2,3-dioxygenase in patients with aGVHD after allo-HSCT[Wiley: source]
Indonesian Journal of Cancer ChemopreventionSecretion of Indoleamine 2, 3-Dioxygenase, an Immunomodulatory Substance, by Adipose-Derived Mesenchymal Stem Cell[Iregway: Source]
PLoS ONECD40 Gene Silencing Reduces the Progression of Experimental Lupus Nephritis Modulating Local Milieu and Systemic Mechanisms[PubMed: PMC3683035]
HepatologyHuman CD14+ CTLA-4+ regulatory dendritic cells suppress T-cell response by cytotoxic T-lymphocyte antigen-4-dependent IL-10 and indoleamine-2,3-dioxygenase production in hepatocellular carcinoma[Pubmed: 23960017]
Free Radical Biology and MedicineIndoleamine-2,3-dioxygenase elevated in tumor-initiating cells is suppressed by mitocans[ScienceDirect: S0891584913006345]
Frontiers in OncologyCancer-Associated Fibroblasts from lung tumors maintain their immuno-suppressive abilities after high-dose irradiation[Frontiersin:Source]
Tissue Engineering Part AImmunogenicity and escape mechanisms of allogeneic tendon-derived stem cells[Pubmed:24813640]
Immunobiology.The expression of dendritic cell subsets in severe chronic rhinosinusitis with nasal polyps is altered[Pubmed:24947894]
ApoptosisChronic hepatitis C virus infection triggers spontaneous differential expression of biosignatures associated with T cell exhaustion and apoptosis signaling in peripheral blood mononucleocytes[Pubmed:25577277]
Front OncolCancer-Associated Fibroblasts from Lung Tumors Maintain Their Immunosuppressive Abilities after High-Dose Irradiation[PubMed: 26029659]
Acta Universitatis TamperensisInsights into the use of adipose stem cells for clinical cell therapy: Novel culturing conditions and characterization of multipotency and immunogenic properties[Handle: 10024]
PLoS OneRespiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2, 3-Dioxygenase[pubmed:27695127]
Journal of Biomedical NanotechnologyExploring Amino Acid-Capped Nanoparticles for Selective Anti-Parasitic Action and Improved Host Biocompatibility[Pubmed:29883557]
BiochemistryMechanisms of Leptin and Ghrelin Action on Maturation and Functions of Dendritic Cells[]
American Journal of PathologyCognitive Decline, Cerebral-Spleen Tryptophan Metabolism, Oxidative Stress, Cytokine Production, and Regulation of the Txnip Gene in 3xTg-AD Mice[Pubmed: 30980800]
international immunopharmacologyInteraction of the immune-inflammatory and the kynurenine pathways in rats resistant to antidepressant treatment in model of depression[Pubmed: 31176083]
Stem Cell ReportsRichard Schäfer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Kuçi, 4[]
International Journal of?Tryptophan?ResearchAltered Indoleamine 2, 3-Dioxygenase Production and Its Association to Inflammatory Cytokines in Peripheral Blood Mononuclear Cells Culture of Type 2 Diabetes?¡­[33343199]
Stem Cell ReportsRichard Sch?fer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Ku?i, 4[]
Transl PsychiatryAssociations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive …[34802039]