吲哚胺2,3-双加氧酶(IDO)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Indoleamine-2,3-Dioxygenase (IDO)
CD107B; INDO; Indoleamine-Pyrrole 2,3 Dioxygenase
- 编号KSB547Hu01
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.312-20ng/mL
- 可适灵敏度0.131ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7182¥ 11970¥ 21546¥ 41895¥ 71820
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对吲哚胺2,3-双加氧酶(IDO)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB547Hu01 | 吲哚胺2,3-双加氧酶(IDO)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB547Hu01 | 吲哚胺2,3-双加氧酶(IDO)活性蛋白 | Cell culture; Activity Assays. |
| PAB547Hu01 | 吲哚胺2,3-双加氧酶(IDO)多克隆抗体 | WB; IHC; ICC/IF |
| LAB547Hu71 | 吲哚胺2,3-双加氧酶(IDO)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAB547Hu81 | 吲哚胺2,3-双加氧酶(IDO)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAB547Hu22 | 吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | WB;ICC/IF;FCM |
| MAB547Hu24 | 吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB547Hu21 | 吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | WB; IHC. |
| MAB547Hu23 | 吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | WB; IHC; ICC; IP. |
| FAB547Hu08 | 抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | Flow cytometry. |
| FAB547Hu06 | 抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | Flow cytometry. |
| FAB547Hu04 | 抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | Flow cytometry. |
| FAB547Hu02 | 抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体 | Flow cytometry. |
| LAB547Hu72 | 吲哚胺2,3-双加氧酶(IDO)单克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEB547Hu | 吲哚胺2,3-双加氧酶(IDO)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB547Hu | 吲哚胺2,3-双加氧酶(IDO)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PSB547Hu01 | 吲哚胺2,3-双加氧酶(IDO)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSB547Hu01 | 吲哚胺2,3-双加氧酶(IDO)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Blood | CCL18 differentiates dendritic cells in tolerogenic cells able to prime regulatory T cells in healthy subjects[Pubmed: 21803856] |
| Clinical Transplantation | Potential immunosuppressive function of plasma indoleamine 2,3-dioxygenase in patients with aGVHD after allo-HSCT[Wiley: source] |
| Indonesian Journal of Cancer Chemoprevention | Secretion of Indoleamine 2, 3-Dioxygenase, an Immunomodulatory Substance, by Adipose-Derived Mesenchymal Stem Cell[Iregway: Source] |
| PLoS ONE | CD40 Gene Silencing Reduces the Progression of Experimental Lupus Nephritis Modulating Local Milieu and Systemic Mechanisms[PubMed: PMC3683035] |
| Hepatology | Human CD14+ CTLA-4+ regulatory dendritic cells suppress T-cell response by cytotoxic T-lymphocyte antigen-4-dependent IL-10 and indoleamine-2,3-dioxygenase production in hepatocellular carcinoma[Pubmed: 23960017] |
| Free Radical Biology and Medicine | Indoleamine-2,3-dioxygenase elevated in tumor-initiating cells is suppressed by mitocans[ScienceDirect: S0891584913006345] |
| Frontiers in Oncology | Cancer-Associated Fibroblasts from lung tumors maintain their immuno-suppressive abilities after high-dose irradiation[Frontiersin:Source] |
| Tissue Engineering Part A | Immunogenicity and escape mechanisms of allogeneic tendon-derived stem cells[Pubmed:24813640] |
| Immunobiology. | The expression of dendritic cell subsets in severe chronic rhinosinusitis with nasal polyps is altered[Pubmed:24947894] |
| Apoptosis | Chronic hepatitis C virus infection triggers spontaneous differential expression of biosignatures associated with T cell exhaustion and apoptosis signaling in peripheral blood mononucleocytes[Pubmed:25577277] |
| Front Oncol | Cancer-Associated Fibroblasts from Lung Tumors Maintain Their Immunosuppressive Abilities after High-Dose Irradiation[PubMed: 26029659] |
| Acta Universitatis Tamperensis | Insights into the use of adipose stem cells for clinical cell therapy: Novel culturing conditions and characterization of multipotency and immunogenic properties[Handle: 10024] |
| PLoS One | Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2, 3-Dioxygenase[pubmed:27695127] |
| Journal of Biomedical Nanotechnology | Exploring Amino Acid-Capped Nanoparticles for Selective Anti-Parasitic Action and Improved Host Biocompatibility[Pubmed:29883557] |
| Biochemistry | Mechanisms of Leptin and Ghrelin Action on Maturation and Functions of Dendritic Cells[] |
| American Journal of Pathology | Cognitive Decline, Cerebral-Spleen Tryptophan Metabolism, Oxidative Stress, Cytokine Production, and Regulation of the Txnip Gene in 3xTg-AD Mice[Pubmed: 30980800] |
| international immunopharmacology | Interaction of the immune-inflammatory and the kynurenine pathways in rats resistant to antidepressant treatment in model of depression[Pubmed: 31176083] |
| Stem Cell Reports | Richard Schäfer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Kuçi, 4[] |
| International Journal of?Tryptophan?Research | Altered Indoleamine 2, 3-Dioxygenase Production and Its Association to Inflammatory Cytokines in Peripheral Blood Mononuclear Cells Culture of Type 2 Diabetes?¡[33343199] |
| Stem Cell Reports | Richard Sch?fer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Ku?i, 4[] |
| Transl Psychiatry | Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive …[34802039] |