武汉云克隆科技股份有限公司

吲哚胺2,3-双加氧酶(IDO)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Indoleamine-2,3-Dioxygenase (IDO)

CD107B; INDO; Indoleamine-Pyrrole 2,3 Dioxygenase

  • 吲哚胺2,3-双加氧酶(IDO)检测试剂盒(酶联免疫吸附试验法)产品包装(模拟)
  • 吲哚胺2,3-双加氧酶(IDO)检测试剂盒(酶联免疫吸附试验法)产品包装(模拟)
  • 吲哚胺2,3-双加氧酶(IDO)检测试剂盒(酶联免疫吸附试验法)实验结果图
  • SEB547Hu.jpg标准曲线图
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测吲哚胺2,3-双加氧酶(IDO)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的吲哚胺2,3-双加氧酶(IDO)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本回收率范围(%)平均回收率(%)
serum(n=5)80-10497
EDTA plasma(n=5)79-10594
heparin plasma(n=5)96-10399

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的吲哚胺2,3-双加氧酶(IDO)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中吲哚胺2,3-双加氧酶(IDO)含量的测定值与理论值的比率。

样本1:21:41:81:16
serum(n=5)82-93%83-92%78-99%89-104%
EDTA plasma(n=5)85-93%80-94%86-104%79-90%
heparin plasma(n=5)96-103%78-93%84-104%87-94%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将吲哚胺2,3-双加氧酶(IDO)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的吲哚胺2,3-双加氧酶(IDO)与连接于固相载体上的抗体结合，然后加入生物素化的吲哚胺2,3-双加氧酶(IDO)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的吲哚胺2,3-双加氧酶(IDO)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号适用物种：Homo sapiens (Human，人)应用(仅供研究使用，不用于临床诊断！)
RPB547Hu01吲哚胺2,3-双加氧酶(IDO)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APB547Hu01吲哚胺2,3-双加氧酶(IDO)活性蛋白Cell culture; Activity Assays.
PAB547Hu01吲哚胺2,3-双加氧酶(IDO)多克隆抗体WB; IHC; ICC/IF
LAB547Hu71吲哚胺2,3-双加氧酶(IDO)多克隆抗体(生物素标记)WB; IHC; ICC.
LAB547Hu81吲哚胺2,3-双加氧酶(IDO)多克隆抗体(异硫氰酸荧光素标记)WB; IHC; ICC; IF.
MAB547Hu22吲哚胺2,3-双加氧酶(IDO)单克隆抗体WB;ICC/IF;FCM
MAB547Hu24吲哚胺2,3-双加氧酶(IDO)单克隆抗体WB; IHC; ICC; IP.
MAB547Hu21吲哚胺2,3-双加氧酶(IDO)单克隆抗体WB; IHC.
MAB547Hu23吲哚胺2,3-双加氧酶(IDO)单克隆抗体WB; IHC; ICC; IP.
FAB547Hu08抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体Flow cytometry.
FAB547Hu06抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体Flow cytometry.
FAB547Hu04抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体Flow cytometry.
FAB547Hu02抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体Flow cytometry.
LAB547Hu72吲哚胺2,3-双加氧酶(IDO)单克隆抗体(生物素标记)WB; IHC; ICC.
SEB547Hu吲哚胺2,3-双加氧酶(IDO)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMB547Hu吲哚胺2,3-双加氧酶(IDO)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
PSB547Hu01吲哚胺2,3-双加氧酶(IDO)抗体对ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSB547Hu01吲哚胺2,3-双加氧酶(IDO)检测试剂盒DIY材料(酶联免疫吸附试验法)Main materials for "Do It (ELISA Kit) Yourself".

参考文献

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Free Radical Biology and MedicineIndoleamine-2,3-dioxygenase elevated in tumor-initiating cells is suppressed by mitocans[ScienceDirect: S0891584913006345]
Frontiers in OncologyCancer-Associated Fibroblasts from lung tumors maintain their immuno-suppressive abilities after high-dose irradiation[Frontiersin:Source]
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